Values are the mean of quadruplet cultures of MSE experiment and duplicate cultures of Stores That Sell Kratom In California MIT experiment. Bars are SEM. ANOVA with Dunnet post test.
Q ANOVA with Dunnet post test. Stores That Sell Kratom In California m) Control 0. Q2 (%) 1.
The cells were Stores That Sell Kratom In California returned to the kratom opiate receptors incubator for another 24 hr and another reading was made at the 48 hr time point. MIT concentrations as described earlier and the cells were incubated for 48 hr time Stores That Sell Kratom In California point. Cell viability was assessed as routine Trypan blue
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exclusion procedure described in section 2. Analysis of MSE using UV-VIS spectrometer A UV-VIS spectrometer (WPA Lightwave II) was utilised for estimating the MIT content in the MSE fraction Stores That Sell Kratom In California samples by measuring UV spectral characteristics of MIT.
Therefore the role of p53 and p21 in MSE and MIT induced toxicity were examined. However
in the present studies the cell cycle arrest noted appeared to be independent of induction of p53 and p21. The loss of the protein was strongly dose-dependant as there was a time dependant induction of p53 expression observed in the control and lower dose groups indicating a normal p53 expression response in this cell line. The effect of MIT on the expression of p53 was also assessed. MIT has demonstrated weak toxicity effects compared to MSE. As anticipated the experiments clearly showed that p53 was still being expressed i MIT treated groups and in control group but down regulated with time- dependant manner. M) the same pattern of p53 down regulations was seen as with the higher dose of MSE.
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Cells were treated for 24 48 and 72 hrs and harvested as described in the methods. Values are the mean of duplicate
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