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Biochemical and morphologic studies of heterogenous lobe responses in hepatocarcinogenesis. Carcinogenesis 7: 247-251. Microinjection of cathepsin d induces caspase-dependant apoptosis in fibroblasts.
From these estimates it appears that the SH-SY5Y cells are the most sensitive of those examined to the cytotoxic and possibly cytostatic effect of MSE. Based upon my estimation of 42% MIT-like compound in MSE extract the SHSY5Y cell IC50 for MSE is equal to 9. M MIT-like compound. This is not dissimilar to the experimentally determined IC50 for pure MIT of 7. To assess the long-term effect of MSE on surviving cells after acute treatment a clonogenicity assay was performed after 24 hr treatment on HEK 293 and SHSY5Y cells. Additional clonogenicity assays using chloroform and combinations of chloroform and MSE were also carried out to determine whether potential chloroform contamination of MSE could influence cytotoxicity. MSE were unable to generate colonies.
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This assay was performed as instructed by the manufacturer Promega USA. Serial fluorescence readings were performed using a plate reader at kratom high seattle 485 nm excitation and 520 nm emission. The SH-SY5Y cells were again used in this assay and the caspase inhibitors purchased from Calbiochem included Caspase-3 inhibitor II (Z-DEVD-FMK) Caspase-8 inhibitor II (Z-IETD-FMK) Caspase-9 inhibitor I (Z-LEHD-FMK) Caspase general inhibitor I (Z-VAD-FMK) negative control (Z-FA-FMK) and positive control doxorubicin HCL.
After 24 hr incubation the medium was aspirated and the cells were washed with PBS. Digital photographs were taken of each well at magnification x400. Two pictures were taken for each well as indicated in the figure 2 above.
Metabolically competent Maeng Da Kratom History Harwood human cell line expressing