However it appears that there was no involvement of the cell cycle protein p53 and the p21 pathway with MSE. This was not the case with MIT. Good Opiate Combinations dose dependant lost of p53 and p21 observed at the same concentrations causing cell cycle arrest remains unexplained.
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Then the lysates were centrifuged at 10000g for 1 minute and the supernatant (cytosol exract) was collected and kept on ice. B(containing 4% cupric sulphate):A (containing sodium carbonate sodium bicarbonate bicinchoninic acid and sodium tartrate in 0. M sodium hydroxide) (Pierce U.
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