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For MIT treated cells (Fig. M showed significant differences compared to control group for all fluorometric readings. For 18 hr incubation time period (Fig. Kratom Forest Stryker b) again there was no significance difference between MSE treated groups and control group. Whereas for MIT as shown in previous 4 hr incubation time point similar results were observed for both MIT treated groups.

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As anticipated the experiments clearly showed that p53 was still being expressed in MIT treated groups and in control group but down regulated with time- dependant manner. M) the same kratom extract how to smoke pattern of p53 down regulations was seen as with the higher dose of MSE. The next experiment was carried out to further investigate if there was a correlation between p53 changes and its target gene p21 in response to MSE and MIT treatment.

Some people report that after using the plant they experience headaches and nausea which usually ceases after a short while. There are some known possible negative effects to kratom use especially after a longer period of regular consumption. In East Asia it is also often used as a substitute for opium when opium is unavailable or to moderate opium addiction. Mitragynine is used to gradually wean the user off narcotics. Within a few days the red vein thai kratom powder addict would stop use of the narcotic they are addicted to and the cravings and withdrawal will be moderated by the binding of mitragynine to the delta receptors.

Methods in enzymology. British Journal of Pharmacology 147: S153-S162. Metabolically competent human cell line expressing five cDNAs encoding procarcinogen-activating enzymes: application to mutagenicity testing.

Arochlor 1254 is known to be a potent inducer of wide range of mixed-function oxidase enzymes (Puga and Wallace 1998; Ryan et al 1977). CYP 2E1 may have a role in activating MSE toxicity. CYP 2E1 is an important xenobiotic metabolising enzymes for human and rodents which is expressed in the liver. CYP 2E1 can metabolise various substrates including paracetamol fluoxetin alcohol caffeine and many others (Tanaka et al 2000). CYP 2E1 inducers for example alcohol.

M for MSE and MIT respectively (Chapter 2). The nature of cell death observed was unknown and to the best of my knowledge there are no reports or information available on Mitragyna speciosa Korth toxicity on mammalian cells. In this study therefore an attempt was made to characterise the MSE and MIT toxicity by looking at cell cycle distribution. Firstly attempt was made to look at the cell cycle distribution in different cell lines using flow cytometry approach.

Based on the literature it was well known that p53 has the ability to induce G1 arrest and its target gene p21 facilitates the arrest (Ko and Prives 1996) by inhibiting the function of CDKs (Gu et al 1993; Harper et al 1993). Therefore the role of p53 and p21 in MSE and MIT induced toxicity were examined. However in the present studies the cell cycle arrest noted appeared to be independent of induction of p53 and p21. The loss of the protein was strongly dose-dependant as there was a time dependant induction of p53 expression observed in the control and lower dose groups indicating a normal p53 expression response in this

cell line. The effect of MIT on the expression of p53 was also assessed. MIT has demonstrated weak toxicity effects compared to MSE.

The result was generated from a single preliminary experiment. After this preliminary experiment optimisation of the assay was conducted as described in section 5. DCFHDA precipitations seen in the preliminary assay which could interfere with the fluorescence readings.

Copyright 2009-2012 www. All rights reserved. Buy Herbal Extacy and Buy Salvia.DTD XHTML 1. For those looking to buy kratom please take a look in the online smartshop. Kratom is the common name for a plant that carries the scientific name: Mitragyna speciosa Korthals. The traditional use of this plant dates back many centuries and of course has its origins in Thailand. In recent times kratom has become popular for recreational purposes because of the pleasant effects the leaves of this plant can have.

This finding suggests that the mode of the cell death of MIT treated cells is dependant on caspase 3 and 7 activation pathway. There were no significant differences in the subG1 population (apoptosis population) between treated groups (caspase 3 inhibitor caspase 8 inhibitor caspase 9 inhibitor and general caspase inhibitor treated with high dose of MSE) and the control and negative control groups. At this stage it seems that kratom tea for withdrawal despite having high MIT content in the MSE the high dose MSE treatment in SH-SY5Y cells does not activate caspase enzymes. This probably could be due to other chemicals that present in MSE Kratom Forest Stryker preventing the activation of caspase enzymes. Cell death of SH-SY5Y cells after kratom pills legal MSE and MIT appeared to be predominantly via apoptosis based on its morphological appearance however biochemically the results discussed above fail to support a caspase mediating event. As apoptosis could follow various pathways and often vary in different cells (Esposti and McLennan 1998 Hetts 1998) this prompted us to further investigate if other pathways could contribute.

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I did well and I did actually scheduled and illegal drugs. I can do anything I want. Where Can I Buy Kratom In New Jersey i have an two agregia cum laude bachelors with a minor on each an MBA and a Ph.

The p21 Cdk-interacting protein Gp1 is a potent dried kratom dosage inhibitor of G1 cyclin-dependant kinase. Cell 75: 805-816. Cell cycle control and cancer.

ASM press Washington DC. Hypothesis: chemical carcinogenesis mediated by a transiently active carcinogen receptor. Extrinsic versus intrinsic apoptosis pathways in anticancer chemotherapy.

BMJ 329: 257-258. BMJ 332: 175-176 Weinert T. The RAD9 gene controls the cell cycle response to DNA damage in Saccharomyces cerevisiae. Science 241: 317-322 Weterings E. The mechanism of non-homologous end-joining: a synopsis of synapsis. DNA Repair 3: 1425-1435.

Similar observations were also noted for H202 MSE and Where Can I Buy Kratom In New what is red dragon kratom Jersey MIT Where Can I Buy Kratom In New Jersey groups. Interestingly the majority of the cells which kratom legal in us were treated with NAC prior to treatment with H202 appeared firmly attached to the bottom of the wells and had normal cell appearance. Brownish precipitations were also noted floating in all wells believed to be the hydrophobic fluorescent dye DCFH-DA.

MSE at any time point. This finding supports the previous p53 results. Parallel experiments were carried out to assess the effects

of MIT on the expression of Where Can I Buy Kratom In New Jersey p21 protein. In the previous section it was noted that there were no major differences in p53 band intensity over the dose range tested compared to the control group implying that MIT does not induce the loss of protein as seen in the MSE treated cells. As with the p53 effects noted previously MIT had little effect on p21 levels (Fig. P21 levels of MSE treated SH-SY5Y cells at different time points (6 12 24 and 48 hr).

Human p53 gene localized to short arm of chromosome 17. A Phase III report of sumatra red vein kratom the U. S Environmental Protection Agency Gene-Tox Program1. Mutation Where Can I Buy Kratom In New Jersey Research 394 177-303.

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As shown in the table 3. MLA Kratom Addiction Bluelight results for MIT in the presence or absence of rat liver S9 show no evidence of genotoxicity. The outcome of this experiment would seem to be contrary to what was seen for MSE.

The limited amount of MIT available super premium indonesian kratom dosage to me throughout the studies have restricted the testing of MIT in parallel with all MSE assessments. Kratom Addiction Bluelight this limitation has compromised a comprehensive investigation on MIT induced cytotoxicity and cell death. It is therefore important for future in vitro investigations to look for morphological assessment Kratom Addiction Bluelight of MIT induced cell death and further confirmation on the involvement of initiator caspases 8 and 9 to support the current findings. MSE and should be supported by in vivo studies. Metabonomic studies using cell lines or urine from animal models or perhaps urine from humans exposed to this plant are also suggested. Analysis of this study is underway.

Great info page. Daniel Seibert in email btwnot just from his website). NO loss of potency whatsoever.

DIABLO in completing the cell death cascade. Mitochondria have also been shown as an important factor in other caspase-independant apoptosis. Generation of reactive oxygen species (ROS) is also a part of the mitochondrial function. Under Kratom Addiction Bluelight normal circumstances the low levels of ROS generated by mitochondria as a normal by kratom military drug test product of oxygen metabolism are usually removed by an abundance of endogenous free radical scavengers such as enzyme superoxide dismutases glutathione and other cellular antioxidants such as ascorbic acid and vitamin E (Yazdanparast and Ardestani 2007; Fridovich 1999). However xenobiotic insult which causes mitochondrial malfunctions may lead to generation of ROS in higher levels thus triggering further serious Kratom Addiction Bluelight problems such as oxidative stress lipid peroxidation and finally cell death.

C 40 30 20

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10 5 MMS Cell conc. X 105 8. Relative suspension growth (RSG) 91.

MSE in this cell line revealed that cell cycle arrest was again noted at 24 hr and more prominent at G1 phase

  • This observation is in contrast of what was seen for MSE pre-treated NAC groups
  • Fundamental and Molecular Mechanism of Mutagenesis 59: 61-108
  • The Mitragyna genus part of the family Rubiaceae is found in tropical and sub-tropical regions of Asia and Africa
  • Resin and powder are usually stronger than leaves but the strength of each product also depends on the age and quality of the plants it was made from
  • Targeting death and decoy receptors of the tumour-necrosis factor superfamily
  • The slides were mounted with DPX and were examined using Zeiss Axiovert 200 widefield microscope at 1000x magnification
  • The same goes for resin

. Again on reflection inclusion macht kratom high of control group for each time points would have aided interpretation of these experiments. Based on what is maeng da kratom powder the results of the three different cell lines examined it is suggested that MSE causes cell cycle arrest at G1 phase and S phase.

Biochemical analysis using caspase enzymes and fluorescent dye 27dichlorofluorescein diacetate (DCFH-DA) for detecting ROS generation in live cells were also conducted to confirm the mode of cell death. And finally the possible involvement of opioid receptors in mediating the MSE and MIT cytoxicity has also been investigated. A diagram showing the extrinsic and intrinsic pathways of apoptotic cell death involving initiator caspases 8 and 9 and executioner caspases 3 and 7.

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The DEA concedes that Kratom has been around for thousands of years. State Senator A. Crowe a Republican from Kratom Gold Reserve Dosage White River Louisiana. Crowe sponsored the first law in the country last year that bans the sale of Kratom to minors. The latest research backs that up. Stop being a wuss.

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The leaves of the tree can be chewed made into a tea or dried and added to capsules that cure anxiety depression migraines and many types of chronic pain. The plant acts as both a mild sedative when used kratom drug of concern sparingly; and in large doses as a stimulant. Error! Please try again. Adobe XMP Core 5.

A bit more expensive but a worthwhile treat once in awhile! I hope this information will help those who are new to Kratom or just on the fence about trying a new vendor. Hi Gina! Thanks so much for taking the time to make the jump over here. I am working on a way to get the previous comments imported to this new blog because I realize what a pain it is to have to navigate between two blogs. I can find a way to notify Colby to head over this way.

They are free of insect damage discoloration and other imperfections. They are dried extra carefully to retain good leaf color. The leaves are then carefully stacked and packaged so that they remain whole and unbroken during shipping. Perfect for ceremonial use. These are the leaves pictured at left.

At strong doses the effects are profoundly euphoric and immensely pleasurable. Typically people describe the effects as dreamy ecstatic and blissful. Many people experience dream-like closed-eye visuals.

The Thai buzz is quit strong and generally lasts about 5-7 hours causing euphoria as well as diminishing or completely taking away restlessness. Many people are astounded by the strength from their very first use. Kratom extracts are basically the purest form of kratom they extract the main alkaloids that cause its powerful effects. These extracts are extremely potent and you need not much to feel the effects. The effects are usually felt at lower doses of 1-2g.

That herb remains our specialty but now we also offer kratom kava kava other herbs books and related products. Before placing an order please read the detailed ordering Kratom Gold Reserve Dosage White River instructions and sales policies at the bottom of this page. Drug Administration (FDA).

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There are short biographies describing each healer and their kratom powder vs resin particular specialties and practices. The back of the book includes a map of the Sierra Mazateca showing the area where each resides. I
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In the traditions of every culture plants have been highly valued for their nourishing healing and transformative properties. He discusses their ritual and medicinal usage cultural artifacts made from these plants and works of art that either represent thai kratom or maeng da or have been inspired by them. Although this book may seem a bit expensive it is actually quite reasonably priced considering its size and the number of photographs that it contains.

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<p>After the <a href=http://www.smoketalk.net/kratom/in/latrobe/pennsylvania>leaves have been strained a second</a> time they can be discapsuleed. Put the combined liquid from both boiling back into the pot and boil until the volume is reduced to about 100 ml. The idea is to boil the tea down to a small volume so that each individual dose can <i>15x Kratom Pills Boy River</i>  be quickly <br /><iframe width="560" height="315" src="http://www.youtube.com/embed/4Km0uaefbHg" frameborder="0" allowfullscreen></iframe><br />  swallowed. You can boil it down to whatever concentration you are comfortable with. Be careful near the end of the kratom tincture withdrawal process. On occasion the foliage is extracted into <b>kratom capsules buy online hawesville</b>  water and evaporated proper tar which sometimes then be swallowed.</p>
<p>It lasted for <i>15x Kratom Pills Boy River</i>  around 2 hours. May be it was a mistake to mix it with cold water (?). I had trouble ingesting it because it tasted really bad and made me want to throw up. It also made me really thirsty.</p>
<p>And in the future purchase can be allowed to make it widely available to more patients around the world. The leaves of Kratom are now easily <b>15x Kratom Pills Boy River</b>  available in the market. <a href=http://orderkratomcapsules.com/?p=8353/>A South</a> East Sector of Asia and Japan. According to the researches these leaves consist of an ingredient known as Alkaloids which has its effect on the mind of the patient. Kratom including pain killers.</p>
<p>A review of herbal marijuana alternatives (kratom kratom) synthetic cathinones (bath salts) kratom Salvia divinorum methoxetamine and piperazines. Schmidt MM Sharma A Schifano F et al. Evaluation of UK-based Websites products and product information.</p>
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Part of the Rubiaceae family or the coffee family mitragyna speciosa or kratom is a tall tree that grows abundantly in Southeast Asia. The leaves of the tree are known to have medicinal benefits particularly in relieving pain increasing energy levels and improving mind alertness. It can also be used as a sedative when taken in large doses. The tree itself is tough branching and usually grows from 12 to 30 feet. It has evergreen ovate-acuminate leaves that measure somewhere around 7 Buy Uei Kratom Online Coldwater inches.

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Aside from that when a person took kratom in its sedative level it does not only remove pain but makes you feel well unlike other pain management alternatives which only benumbs you for a while but keeps you worried about when the effects have faded. Kratom makes you feel relaxed and secured. It may also be a great help for keeping your mind and body relaxed so that it can easily combat pain and other general illnesses. It can also boost immune system and prevent cancer and leukemia. In terms of negative side effect kratom do have its own too.

Surely kratom will top the list of alternative pain management solutions. It was in 1940s when kratom was growing in popularity as opium increases its costs and withdrawals are hard to tolerate. They found about the tree that can easily reduce and eliminate all of the withdrawal effects of opium such as headache chills muscle pain depression and others. However in 2010 there was a proposal to lift kratom from being illegal as it does not do any harm to humans in any means. Kratom in lower dosage is more stimulating kratom legal status eu than sedating feeling of sociability increases more talkative and friendly mood.

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us who are also interested in learning more about this exotic South East Asian plant and possibly even thinking about buying some kratom to give it a try for yourself yet are still in that process. FDA is pushing for a nation wide ban on this healing plant medicine it is still legal to purchase in the United States. The kratom tree is actually very closely related to that of the coffee tree and at lower doses produces similar stimulating effects much like a small cup of coffee or espresso but without that crash.

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Cells pre-treated with anti-oxidant NAC produced lower ROS levels than cells treated with H202 alone. Cells treated with both high concentrations of MSE (Fig. Experience Kratom Black Label Capsules Blockton a) and cells pre-treated with NAC appeared similar to Control group. This infers that MSE did not generate ROS which confirmed the earlier finding.

Something else to think around . X 50X . X Kratom extracts.

In vitro antioxidant and free radical scavenging activity of Cyperus rotundus. Journal of Medicinal Food 10: 667674. N-acetyl-L-cycteine affords protection against lead-induced cytotoxicity and oxidative stress in human liver carcinoma (HepG2) cells. Public Health 4: 132-137. Sequential reduction of mitochondrial transmembrane potential and generation of reactive oxygen species in early programmed cell death. A diverse family of proteins containing Tumor Necrosis Factor lucky kratom liquid dosage receptorassociated factors domain. The Journal of Biological Chemistry 276:2424224252.

Therefore it was assumed

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that the minor contamination of chloroform in both MSE and MIT was not contributing to the toxicity. We observed that MSE exerted dose dependent cytotoxicity with several human cancer cells both via trypan blue exclusion assay and clonogenicity assay. Most xenobiotics undergo metabolic activation in the process of exerting their cytotoxicity effects. Cytochrome kratom powder x15 dosage P450 oxidative enzymes are key enzymes involved in this xenobiotic metabolism. To the best of my Experience Kratom Black Label Capsules Blockton knowledge apart from biotransformation of MIT in the fungus helminthosporum sp.

Cancer Research 63: 1846-1852. Identification of opioid receptor subtypes in antinociceptive actions of supraspinally-administered mitragynine

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in mice. Caspases: Enemies within. Science 28: 1312-1316. Herbal medicine research and global health: an ethical analysis.

From the result (Fig. DED a CYP 2A6 inhibitor also gave some protection against MSE and MIT toxicity but was not kratom herb store effective as ATZ. M of ATZ for 48 hr treatment.

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M naloxone was found not sufficient to inhibit the MSE toxicity at the same concentration used for previous experiments. M did give a positive response. Effects of naltrindole on MSE and MIT treated cells: The effects of naltrindole on acute treatment (Fig. M concentration also gave some

protection against MSE toxicity at high dose but not sufficient to be significant when compared to buy kratom quebec Control groups. D) it appears that naltrindole again successfully inhibited MIT toxicity at all concentrations tested.

SG (mean control SG) X 100 Based on the RSG value obtained the concentrations chosen for the plating (viability assessment and mutant frequency) includes at least one dose level with an RSG value of 10-20% a no effect dose and a minimum of two further doses between this range of concentrations. CM10 media was prepared in sterile universal bottles. Experience kratom dosage for opiate withdrawal Kratom Black Label Capsules Blockton The procedure was done under subdued light due to TFT sensitivity to light.

Kratom is a tree native to Southeast Asia. Its botanical name is Mitragyna speciosa. Kratom is in the same family as the coffee tree (Rubiaceae).

The procedures were as described in section 4. Human embryo kidney- HEK 293 cells Using HEK 293 cells the effects of various concentration of MSE on the cell cycle profile was determined at 24 and 48 hr time period (Fig. The 10000 events were collected during the acquisition and the phases of Experience Kratom Black Label Capsules Blockton the cell cycle were gated manually using CellQuest Pro software.

Kratom Legal In South Carolina Rozel

This kratom tea preparation starts by boiling fresh leaves for green-vein borneo kratom powder a minimum of two Kratom Legal In South Carolina Rozel hours and then rapidly cooled by placing the pot into cold water. Kratom Legal In South Carolina Rozel this rapid cooling gives the Air Ketum a visually appealing chill haze and prevents the hot liquid from kratom glycerin tincture melting the plastic cups and burning the hands of the customers who buy kratom experience reports kratom tea. According to reports children as young as 13 buy Air Ketum on their lunch breaks and before school. To protect the future of this amazing leaf we ask that you use it does kratom affect fertility responsibly.

As of October 2 2013 the justice ministry of Thailand suggested removal of kratom from the narcotic drug list relating to Category 5 of the Narcotic Drug Law of 1979 though still recommended regulating kratom in other ways due to its effects on the nervous system. This recommendation will be made to the Ministry of Public Health which can move forward with the removal from the list or not. Malaysia under Section 30 (3) Poisons Act 1952 and the user may be penalized with a maximum compound of MYR 10000 (USD 3150) or up to 4 years imprisonment.

For instructions on adding this tag and more information on the above requirements read the setup guide: google. Catalog ul li. Custom gaready event – no need to check if ga is ready. Methods attached after gaready has been fired will execute immediately.This article has multiple issues. Inconsistent use of kratom 50x powder review sources.

The most famous is Full Spectrum Tincture (FST). All the exciting bits of kratom in a glass vial of around 2-5mL. FST each time it was dipped into the vial.

OPMs GOLD Kratom – O. Extract Capsules 2ct. Featured Items Products On Sale New Releases Best Sellers Latest Blog Posts Thank you for your vote.

Herbal Eye Kratom best kratom for depression extracts are the best selling all natural Kratom product available in the UK. We utilise a pioneering extraction process which makes our Kratom extract the highest quality Kratom available. Kratom extract provide a concentrated form of the active alkolides that are present in the Kratom plant. The mildest of all our standardise Kratom extracts contains 15g of Kratom per 1g of extract. This standardised extract has 30g of kratom and its active alkolids per gram of extract. The most popular kratom extract at 60 times the concentration of normal Kratom.

Lyophilized Thai Kratom Extract is made from an organic extraction process and then filtered to remove all impurities such as fats and chlorophyll. From there it is Lyophilized or freeze dried which removes all moisture and produces one of the purest Kratom products on the market today. This full spectrum Kratom Tincture is the most potent incense extract available.

Its about as dangerous as marijuana in the fact that you can just simply take it for years and then stop with only very minute discomfort honestly quitting caffeine is more hardcore. I am glad to see that people are learning about the plant and how safe it actually is as any place it gets banned they look into it and amend the bill. This article makes me sick.

Kratom Extract Experiences Iron Mountain

I was told about kratom and started taking it. I was able to come off Suboxone with an extremely light detox and have been taking kratom ever since for 5 years with no relapse. Kratom saved my life I have a functional life my family wants to be around me now and I am off all of my 12 RX medications which consisted of antidepressants Xanax and many other anti-anxiety neds and a couple other types. Kratom Kratom Extract Experiences Iron Mountain Extract Experiences Iron Mountain i use kratom to keep me away from heroin and alcohol.

Worst thing that happened was I got shakey and nauseous. Kratom is a wonderful gentle herb that has helped me immensely with my depression and my shyness in social situations. What Kratom Extract Experiences Iron Mountain a load of crap. In fact I have heard numerous stories of people with opiate addiction using kratom as a clutch to get through the withdrawal process.

ALLOW 3-5 Days even if using EXPRESS MAIL. The USPS cannot be trusted and can you snort maeng da kratom they are soon to be obsolete entity anyways. See? Not a bad review. EXCELLENT compare to the other sites selling this wonderful blessed plant. Reasonable Price fair and less expensive in MOST cases Excellent Product. I repeat: EXCELLENT PRODUCT IF you prefer the stimulant over the sedation. The sedation kicks in the middle and end.

I am only describing what I would do if I were in your situation. It should be easier to get off of Kratom than Oxy. I do not know how much Kratom you thai essence kratom take but I would start staggering the doses then I would wean myself off slowly.

I am glad it has helped you but kratom extraction vinegar your company is not helping. It wouldnt surprise me if your hole story there is just made-up BS to help you sell more Kratom and bring in more profits. I want to make one thing perfectly clear! I really respect an editor like 15x kratom extract yourself not falling for the pharma and giving kratom a second chance! I was totally convinced that you were completely sold out to pharma. ultra enhanced indo kratom review The day that this article and the minute this was originally published I was about ready to board a plane to Seattle Washington standing in the Daytona Florida airport.

The herb has been shown in some individuals to lower blood pressure balance cholesterol levels and reduce blood sugar. Importantly the supplement has been discovered to have antioxidant properties. Antioxidants clear the body of toxins and slow oxidation the process that causes aging.