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P53 levels of MSE treated SH-SY5Y cells at different time points (6 12 24 and 48 hr). P53 levels of MIT black ice kratom side effects treated SH-SY5Y cells after 24 hr treatment. P53 levels of MIT treated SH-SY5Y cells at different time points (6 12 24 and 48 hr). Effects of MSE and MIT on p53 target gene product p21 It is well green malay kratom buy schroeder established that induction of p53 can lead to expression of target gene p21 and thereby cell cycle arrest.

This phenomenon creates disadvantages for this assay as when the whole FACS profile shifts to the right side of the scale the determination of the stages of cell death is difficult to interpret as the cells are no longer located in specific quadrants. This observation is clearly in contrast with the previous cytological examinations which indicated that SH-SY5Y cells treated with high dose of MSE undergo apoptosis rather than necrosis. The right shifting phenomenon for MIT treated cells observed in fig.

Control 1 10 50 100

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250 91. Q2 (%) 3. Q4 (%) 0.

Carcinogenesis 7: 247-251. Microinjection of cathepsin d induces caspase-dependant apoptosis in Captain Kratom Premium Thai Capsules fibroblasts. Cathepsins as effector proteases in hepatocytes apoptosis. Wound- healing assay.

Thus the findings of this study will hopefully contribute to a better understanding in predicting the risk upon consuming Mitragyna speciosa Korth leaves. M human consumption of Mitragyna speciosa Korth leaves at pharmacologically active doses would appear to be substantially lower than the threshold of toxicity predicted from my in vitro study. Taking into account all the findings of my studies MSE and MIT could be potentially harmful in humans at high doses. The safety assessment assumptions suggest that the use of kratom extract oil blauvelt Mitragyna speciosa Korth leaves within the range of pharmacologically active doses as reported in the literature is probably safe however caution should be taken as MSE toxicity in this study was found to be enhanced by metabolism particularly by CYP 2E1. Thus the combination consumption of Mitragyna speciosa Korth leaves with CYP 2E1 inducers may shift toxicity closer to doses that are pharmacologically active.

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In the absence of rat liver

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S9 (Table 3. MIT was reduced to 17% of the concurrent vehicle control implying excessive toxicity effects. This was due to the measured RSG value being very low (18. A) which therefore affected the final calculation for the RTG. Preliminary data of MIT treated groups with and without the presence of S9. C MIT Treatment with S9 (3 hr) 30 20 10 5 DMBA Neg.

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