To date there is no information or report on cancer or tumour kratom 15x testimonials incidence in humans consuming Mitragyna speciosa Korth leaves. It is important to find out whether MSE and MIT cytotoxicity is accompanied by DNA damage. Buy Kratom Extract Mc Cleary this chapter examines whether MSE or MIT have genotoxic potential and thereby the potential for carcinogenicity.
Effect of MSE and MIT on p53 protein levels SH-SY5Y a neuroblastoma cell known to have wild type p53 (Moll et al 1995 1996) was examined by immunoblotting as described in section 4. Image J version 1. The
effects of MSE on p53 expression levels were assessed.
A and B a similar pattern of results was noted as in the preliminary assay (Fig. Again the positive control group H202 treated cells in both experiments seems
to generate higher ROS levels compared to other groups. Cells pre-treated with anti-oxidant NAC produced lower ROS levels than cells treated with H202 alone. kratom tea no effect Cells treated with both high concentrations of MSE (Fig. A) and cells pre-treated with NAC appeared similar to Control group. This infers that MSE did not generate ROS which confirmed kratom resin effects the earlier finding.
SG) for 2 days expression Buy Kratom Extract Mc Cleary period were calculated and SG of each test cultures were compared to control. SG (mean control SG) X 100 Based on the RSG value obtained the concentrations chosen for the plating (viability assessment and mutant frequency) includes at least one dose level with an RSG value of 10-20% a no effect dose and a minimum of two further is kratom tincture good doses between this range of concentrations. CM10 media was prepared in sterile universal bottles. The procedure was done under subdued light due to TFT sensitivity to light. Scoring the plates After the incubation period all the plates for viability assessment were scored using a modified mirror box for the absence or presence of colonies in each well.
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